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DR1and DR2 had very low flavonoid content.
However, extract I with somewhat low flavonoid content had high antioxidant and peroxide scavenging activities (Table 3).
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The highest flavonoid content was in chrysanthemum extract, whereas the lowest flavonoid content was observed in bael extract.
Like TPC fractionation, n-hexane also proved to be the lowest flavonoid content bearer in this study.
The lowest flavonoid content was seen in the chloroform fraction (1.1 mg RE/g of dry powder) and the highest was seen in the methanol fraction (3.3 mg RE/g of dry powder).
Compared to conventional extraction (CE-NF, 6.69 mg GAE/g), the phenol amounts of HPE-BLF and HPE-LRF were significantly increased to more than 8 mg GAE/g, while the lowest flavonoid contents were observed for HPE-BLF (0.44 mg RE/mL) and HPE-LRF (0.45 mg RE/mL) (p < 0.05).
As opposed to this, fractions with high biflavonoids level and low flavonoid glycosides content (I/3, I/5) exhibited significantly lower activity (i.e. higher IC50 values), and fraction I-4, practically devoid of flavonoids and phenolic acids, was the least active.
The lowest total flavonoid content was observed in S. halimifolium (0.23 mg QE/g extract).
The presence of low tannin and flavonoid contents in flower could have been contributed to medium low TPC and free radical scavenging activity.
This may be the reason that duckweed has low lignin percentage but high flavonoid content.
On the other hand, moderate correlations were obtained between DPPH and total phenolic content and total flavonoid content indicating low capability to scavenge free radicals, whereby total phenolic content and total flavonoid content exhibit correlation coefficient with DPPH (r = 0.762, r = 0.691, resp).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com