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Therefore, a) we do not recommend to perform amplification from less than 250 pg of input RNA, and b) when it is possible, researchers should try to increase the RNA input quantity to at least 500 pg to be more confident in the biological interpretation of the results, particularly concerning low express transcripts.
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Previous studies suggest RNA-Seq performance was mixed for detecting the differential expression of low expressed genes [ 1, 13].
Increased variance indicates that there is a larger difference between gene expression levels of high and low expressed genes and reflects an increased range of gene expression.
Combination endocrine therapy was superior to CMF in patients with high p27Kip1 expression but not in low expressing patients.
This means that genes with high or low expression in N are also high or low expressed in PRE.
Low expressed genes are detected with insufficient reliability and expression of many genes is never detected at all.
Color scale of the heat map depicts red as the highly expressed, green as low expressed and black as intermittent level of gene expression.
For describing the expression value, three levels are defined as low expressed (100 reads or lower mapped), medium expressed (100 500 reads), and highly expressed (500 or more reads).
Filtering by a minimum expression level obscures the influence of extreme variability of low expressed genes.
Genes that have expression levels less than 1 CPM were labeled as low expressed.
Other low expressing genes included ZYX and RAC1 (cell lines) and DSCAM expression was absent in tumors.
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CEO of Professional Science Editing for Scientists @ prosciediting.com