Sentence examples for low cot from inspiring English sources

An alternative method of normalising clone selection, which has been used by others, is by reassociation of single-stranded library plasmids at relatively low Cot to remove highly expressed clones [ 22, 23].

Following hybridization to a relatively low Cot of 5-10, the remaining single-stranded circles (normalized library) are purified over hydroxyapatite (HAP), converted to double-stranded circles by primer extension and electroporated into bacteria [ 172].

Biotinylated driver RNA produced from the T7 RNA polymerase promoter and single-stranded (ss) target DNA produced from the F1 ori were hybridized to each other at a low Cot (concentration of driver times the time of hybridization) value.

While the importance of the stopping vortex to swimming with a low COT is now recognized, the details of how the stopping vortex, which originates midway up the body on the exumbrellar surface, comes to reside at the animal's subumbrellar surface and exhibits high vorticity remains undescribed.

No regions of obvious differential Cot-1 RNA FISH levels were observed in nuclei with low Cot-1 signal with the exception of one to three strong punctate foci (Fig.  5b).

As a result, at 1.38 m/s of the walking speed, the COT of 1.76 which is the lowest COT through the experiment was achieved.

This value is not so far from current lowest COT of 1.76.

We also plotted the lowest COT of 1.2 of TITAN-XIII with additional 5 kg of weight at 0.83 m/s of the walking velocity in Fig. 21.

The polynomial function of COT vs. speed was calculated and the lowest COT was found where the first derivative equals zero.

The Uopt (i.e. the speed at which the lowest oxygen uptake per unit distance swum, or lowest COT, occurred) was determined according to Palstra et al. [21].

In order to identify more repeats, Hřibová et al. [ 5] applied a low-Cot DNA isolation technique to characterize highly repetitive fractions of banana genome.