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Targeted analyses require significant method development but provide a means for absolute quantitation of proteins with a low coefficient of variance [69]-[71] [69]-[71]
The raw signals obtained for single-color CY3 hybridization were normalized to a stable endogenous control, miR-454, which was chosen due to its low coefficient of variance (CV) and positive signal in the microarray.
We selected the reference gene based on its very low coefficient of variance (0.10) in FPKM values across all sample groups and being the only transcript for its gene component.
The technical assessment experiment shows that data generated by the Lung Cancer DSA is highly reproducible and exhibits extremely good correlation and low coefficient of variance, similar to those obtained from generic Affymetrix arrays in our own [See Additional file 4] and in previously published studies [ 50].
The remaining candidates were selected based on 1) low coefficient of variance in microarray data of a subset of the samples; 2) as low a correlation as possible to clinical parameters that could be of interest (symptom, diabetes, statins, time between symptom and operation, HbA1c values, serum cholesterol); and 3) mean expression level above some fraction of the mean of all probe sets.
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The low coefficients of variance of both the HBV and SIV standard stock results indicate the excellent reproducibility of the single-tube reagent.
Reference genes are identified as the most stable genes, i.e. those that exhibit the lowest coefficient of variance and standard deviation [ 120].
Formaldehyde had a mean and median personal concentration of 21 μg/m (cancer risk = 276 × 10−6), and the lowest coefficient of variance (28%) for all the evaluated HAPs after the exclusion of a relatively high personal measurement (144 μg/m) that was inconsistent with the corresponding indoor concentration (26 μg/m).
Microarray data for the twenty genes with the lowest coefficients of variance were input into Normfinder [ 80].
In particular, strong additive genetic effects of genes for fitness in males will maintain the potential for indirect sexual selection, even if none of these effects are correlated with the effects in females, in case females have a low coefficient of additive genetic variance in fitness.
Low control coefficient of variance (CV) was 6.7% and high control CV was 4.9%.
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