Sentence examples for low cell passage from inspiring English sources

All assays were conducted using low cell passage cells (2 5 passages).

To specifically address the latter issue we here studied the BMP expression profile in cell clones derived from the respective type of tumor and, to ensure that the expression pattern found mimics that of the original cell from the primary tumor, BMP expression patterns were studied at low cell passage numbers.

The human myeloid leukemic cell lines KG-1 and Kasumi-1 were obtained from the American Type Culture Collection (ATTC) and frozen in aliquots at low cell passage.

The mosaic distribution of mtDNA depletion was also apparent at low cell passage number (Supplementary Material, Fig. S1), where occasional mtDNA-free (rho zero) cells were present (Supplementary Material, Fig. S1), interspersed with cells with apparently normal or near normal mtDNA levels.

Low cell passages (between 10 and 20) were used for this study.

High cell passage number has been shown to increase the risk of alterations in DNA fingerprinting and best practices suggest cell identification at low passage numbers [ 44].

Low passage cells (p2) were transduced in the same manner as immortal cell lines.

Low passage cells were grown in 10 cm plates, transfected as above and grown to confluence.

Low passage cells were frozen down at 1 × 106 cells/ml in 10% dimethyl sulfoxide (Sigma) and 50% fetal calf serum (Hyclone).

Cell line studies demonstrate that low passage LNCaP cells (hormone-sensitive prostate cancer cells) have low basal Akt activity, possibly due to early-stage prostate cancer relying on hormones for growth and survival.

To study this, we analyzed a panel of low passage cell lines derived from primary and metastatic lesions in addition to well-established melanoma cell lines.