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These advances show that SRM can be leveraged to interrogate a suite of important physiological processes in species present at low cell counts when peptides specific to the species and process of interest are known.
Through applications of SRM to samples containing some realistic cellular ratios of bacteria and phytoplankton, and with peptides previously characterized on a mass spectrometer, we assessed the ability of targeted proteomics to detect selected metabolic processes of an organism present at low cell counts.
Low cell counts precluded gene expression studies.Cilengitide was well tolerated but had no detectable clinical activity.
The level of soluble CD44 in RA synovial fluid was elevated only in fluids with low cell counts (less than or equal to 7,000/mm3), and not in RA synovial fluid with higher cell counts.
Also related to low cell counts, we were unable to assess effect modification of the association between urban/rural location of injury and mortality by either ISS or transfer status in regression analyses.
Nevertheless, whereas opportunistic infections (OI) are frequent in HIV-infected individuals with low cell counts, OI are uncommon in ASCT patients.
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Detection of target cell inoculations with a low cell count is critical, since enterohemorrhagic strains of E. coli require an infective dose of as few as 10 cells (Schmid-Hempel and Frank, 2007).
In our study, neither CD4 T-cell count (even for patient with very low cell count as <50/mm3) nor anteriority of HIV diagnosis, viral load, HAART at admission, reason for admission, and immunovirological status were significantly associated with ICU mortality.
Furthermore, these analyses were performed only for CVD and for men and women combined, due to low cell count.
5-year survival rate was 44.6% in high cell count group versus 22.5% in low cell count group (P = 0.021, Figure 2D).
Median survival time was 35.1 ± 10.8 months for the high cell count group and 20.3 ± 3.0 months for the low cell count group, respectively.
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