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The mitochondrial genome has a high copy number in comparison to the nuclear archive of DNA; there are potentially 1,000s of mtgenomes per cell, which enables easy detection of important biomarkers, even when only low amounts of samples are available.
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By contrast, because nanoLC separation is sensitive, the analysis can be performed with low amounts of sample.
This involved the injection of low amounts of sample (equalling 1 µg digest per protocol) to prevent column overload and considerable overrun of the dynamic range.
Library preparations starting from low amounts of sample DNA and protocols using single strand ligation procedures can be considered the most prone to contamination.
Owing to this problem, the predictive quality was only level D. Despite a low amount of samples excellent NIRS-models could be developed for lignin (level A).
In the enrichment of glycopeptides, HILIC is more efficient than the generally used agarose bound lectins in the nano-scale analysis because of its smaller particle size (10 µm vs 200 300 µm), which is consistent with the extremely low amount of samples from 2-DE spots.
Thus, the low amount of samples hinders the analyses in these studies and larger genome-wide association studies are required in order to identify novel SNPs associated with azoospermia.
nd, not determined because of the low amount of sample.
The FTIR of SBN1 was not recorded due to the low amount of sample available.
Identification of binding media involves several analytical problems mainly related to the very low amount of sample available and its heterogeneity, and very low content of organic media components as well as unknown state of alteration/degradation.
However, an option for increasing quality of quantification in case of low amount of sample and missing replicated samples is to divide one sample into technical replicates and use more sensitive approaches like 2D fluorescent difference gel electrophoresis (2D-DIGE).
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