Sentence examples for low amount of input from inspiring English sources

As bisulfite modification causes DNA degradation, low amount of input DNA will be resulted in the failure of unbiased amplification.

The low amount of input RNA ranging from 500 picogram to 5 nanogram allows analysis of biopsy derived tissue samples and the small amplicon size of 100 base pairs is compatible with partially degraded FFPE-tissue [ 12].

The availability of only low amounts of input nucleic acid is common, and methods tailored to low-input library generation prior to NGS are required.

Our indexing strategy is compatible with low amounts of input DNA, less than 100 ng, which is often a limiting resource in large populations of DNA samples.

Since we had very low amounts of input DNA we increased the number of cycles in the enrichment PCR to 20.

In vitro transcription of cDNA for the amplification of RNA is a commonly used method that requires low amounts of input total RNA, typically less than 1 μg, often as little as 100 ng.

When the proportion of unique library molecules is low due to inefficient molecular reactions or low amounts of input material, sequencing more reads of that same library would not yield any additional information, but only extra copies of previously sequenced molecules (duplicate reads).

However, limitations in the sensitivity to detect methylated alleles are expected with low amount of DNA input DNA from LCM microdissected primary tumor cells.

A study of intermittent mixing regimes suggests that mass transfer limitations of enzymatic saccharifications at high-solids loadings are significant but can be mitigated with a relatively low amount of mixing input.

Initially, mutations with a low allele fraction owing to only being present in a subset of tumor cells may be missed by liquid biopsies, as the low amount of DNA input to the assay is compounded by the low incidence of the mutation.

Poor sensitivity was also observed when conducting experiments using Nugen WT-Amplification™ system using the lowest amounts of input RNA (50 pg and 100 pg), suggesting that the minimum input limits of the protocol had been reached.