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Cadel Evans, a further three seconds back, also lost significant time to Nibali, while Robert Gesink lost over four minutes and is no longer a factor.
Although he was able to recover to the pits, he had lost significant time on the leaders and fell to fourth.
Schleck started Saturday's time trial in second place, 1 24 behind Sastre, but he lost significant time and was passed on the road by Sastre, who had started three minutes after him.
He lost significant time in stage 14 on the Plateau de Beille, however, ceding six minutes to the race's top riders and falling to 18th.
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Any thoughts that Evans would lose significant time in the race though were tempered by the actions of Wiggins, who also had to change his own bike on the descent, although it is unclear whether he too suffered a puncture.
LeMond experienced difficulty on the first climb and he cracked on the Col de Tourmalet, losing significant time to Claudio Chiappucci, and eventual winner Indurain.
2 Children with asthma are likely to have more days off school, with adverse effects on academic performance, 3 and their caregivers may lose significant time from work. 4 Evidence is growing that symptoms of asthma can be aggravated or triggered by adverse aspects of the indoor environment.
In the Donor 1 study, all mice lost significant weight between the time of transplant and nephrectomy, except the medium-dose group, which also showed the best blood glucose control.
(See Newspaper Magnate Black Resignss In Payments Probe").. Publishers McClatchy and The New York Times have also lost significant share value over the past year, as well as reducing their staffs, and could be next to file for bankruptcy as the ad market outlook remains far from promising.
But at the same time, the magazine has lost significant market share and seen profits decline.
But the prime minister lost significant support among the ethnic Malay majority and the post-election period has been a time of score-settling and political arrests.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com