We first removed the TBCC N-terminal spectrin domain to generate TBCC-C (residues 100 267); this mutant showed a 38% decrease in kcat when compared to wild type TBCC (Table 3; Figure 6F), and lost the robust αβ-tubulin independent activation of GTP hydrolysis.
Indeed, the long history of Roman Catholicism, where Puritanism has steadily lost out to robust earthiness, and doctrinal rigidity has regularly bent before the pressures of lived experience, is itself reason to think that Notre Dame University has found the truest Catholic response to the world's present moment: its brave decision to honor President Barack Obama.
Leslie Lefkow, an Africa researcher for Human Rights Watch, said, "There was definitely a lost opportunity for a robust intervention in 2004, when the situation was clearer in terms of the number and nature of the armed groups".
As you will see when you play around with different assumptions, while the model can tell us something about the most likely outcomes, there is a chance that the election will not go to type and that Mr. Obama will lose decisively despite robust economic growth next year, win under circumstances that look bad for him, and so forth.
We've lost the civilised art of robust, well-informed debate, and we need to get it back.
The Islanders had lost again, and Kyle Okposo, a robust 20-year-old right wing in his first full N.H.L. season, trudged wearily through the locker room.
David Dewhurst, the state's lieutenant governor during more than a decade of robust economic growth, lost to Daniel Patrick, who accused him of being insufficiently flinty.
But even as the show quietly subverts the convention of the monographic exhibition, his work almost never gets lost — it is formally too robust, or as he might have put it, too viscerally plastic.
Unlike much of country music, which expresses disappointment in life and love with a twang of resignation and a nod to alcoholism, soul tends to convey stories of shattered dreams and lost love with a more robust upbeat.
The Samoan, Tongan, and Tahitian languages were never lost, and thus are also fairly robust.
