As in preference aggregation, the cost of relaxing independence is the loss of strategy-proofness.
To gain further insight into the early functions of XMeis3, we followed a gain- and a loss-of-function strategy.
In the loss-of-function strategy we made use of an antisense morpholino oligonucleotide (reviewed in [42] and references therein) to inhibit the translation of XMeis3 mRNA (MOXMeis3).
Choice of loss-of-function strategy and prediction of whether the lncRNA acts in cis or in trans should be informed by its cytoplasmic, nuclear or chromatin localisation.
The in vitro loss-of-function strategy was extremely helpful to analyse the molecular and cellular properties of knock-out murine ESCs leading to embryonic lethality in vivo.
A gain-of-function rather than a loss-of-function strategy was used as this approach does not disrupt gene expression, avoids issues of gene redundancy and allows screening to occur in a primary generation.
These results prompted us to further confirm whether the reversal of tumor-induced T-cell senescence mediated by Poly-G3 and ssRNA40 occurs through TLR8 signaling, using a loss-of-function strategy with siRNA (Peng et al, 2005, 2007).
Here we report the functional analysis of ZFP252 using gain- and loss-of-function strategies.
Gain- and loss-of-function strategies using transgenic over-expression and targeted ablation of candidate genes in in the mouse have provided important mechanistic insights into cardiovascular development, physiology and disease.
In order to test a potential role for Sdf1 in the temporal control of the Fgf pathway, we sequentially used gain- and loss-of-function strategies.
By using both gain-of-function and loss-of-function strategies to selectively activate or inhibit EphA and EphB receptors both in vivo and in vitro, we uncovered the involvement of Eph receptor signalling in the structural plasticity of Purkinje cell spines in the adult cerebellum.
