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Because of this loss of compounds during the polymerization process, reactions of this type are often called condensation reactions.
Since agitation was performed for 8 h, equilibrium could be assumed, and much lower measured PAH concentrations than calculated ones might be attributed to a loss of compounds during elutriate preparation.
This shows that loss of compounds during the analytical process was negligible.
Furthermore, this system utilizes water free eluents, thereby allowing evaporation of solvents at relative low temperatures and thus minimizing the risk of a loss of compounds with low vapor pressure.
We present evidence for strong structural trade-off between robustness – ability to sustain gain or loss of compounds without extinction – and elongation of carotenoid metabolic pathways in species that depend on external (dietary) starting points for their carotenoid production.
It was confirmed that the decrease in potency after fractionation was not due to loss of compounds in the fractionation step as the initial potency was recovered in the pooled NP fractions (data not shown).
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The vial will therefore not be opened prior to preparation of stock solution in order to minimize the risk for possible loss of compound due to differences in air pressure and/or static electricity.
We have tested such conditions previously and found that the presence of ≤10% DMSO helped to reduce the loss of compound to tubes and surfaces in SPR without significantly affecting the binding affinity or the DNA properties on the surface.
In CETA the difference in partitioning of radioactivity between the two compartments is not due to disproportional loss of compound adhering to the plastic ware of the apical and basolateral compartments, since in cell free blank insert experiments an equal percentage of the initial concentration added was absorbed to each compartment and an 80% recovery was obtained after 48 h (data not shown).
In this model, the entire mass of each chemical entering the system during the shower event is assumed to partition between the water and air phases under equilibrium conditions; loss of compound from the shower system by air exchange is ignored.
Capitalizing on evolutionary lability of carotenoid compounds and enzymatic reactions across phylogeny of our study species (Additional file 4: Figure S1 and Additional file 6: Figure S4, Methods) we categorized gains and losses of compounds (Additional file 7: Table S2) in relation to their topological position within the biochemical network of carotenoids (Additional file 5: Figure S3).
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