We observed a peptide-induced functional antibody response that, remarkably, exceeded the response induced by the native protein in outer membrane vesicles, without losing specificity for related strains.
Protein detection methods lose specificity when the protein target is immersed within a matrix of thousands of molecules having wide ranges of concentrations.
Regarding epitopes on one receptor, nanocarriers addressed with anti-TfR-R17 maintained brain and lung targeting in mice, compared with "free" antibody, while anti-TfR-8D3 nanocarriers lost specificity.
The anti-tumor activities and safety of TCR-transferred T cells can be improved by knocking out endogenous TCR, which avoids the use of affinity-enhanced TCRs that may lose specificity and cause severe adverse effects.
In a press release, Girirajan explained, "When individuals carrying classically-defined genetic syndromes were evaluated for features of autism, a high frequency of autism was observed, even among disorders not previously associated with autism, suggesting that the tools for diagnosing autism lose specificity when applied to individuals severely affected by other genetic syndromes".
Thus, based on the EC50 of the ER agonists, the dose chosen would be about 50 100 times this EC50 and thus should be at high enough doses to saturate their respective ER but not so high as to lose specificity.
Together, these results show that LHP1-CD* lost specificity for H3K27me3 in vitro and that LHP1-CD*-GFP cannot bind to at least some LHP1 targets in vivo, which may explain its altered sub-nuclear localization.
In previous work [20], [21], [48], we presented implementations of multicompartment systems and resolved the problem of readjusting vesicle formation/composition as well as of losing specificity by using linkers consisting of biotinylated DNA single strands (biotin-ssDNA) that were anchored by long and flexible phospholipid-grafted biotinylated PEG tethers via streptavidin as a connector.
This enhances sensitivity without losing specificity.
