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The CFSE dye is inherited by daughter cells after cell division and each dividing cell loses fluorescent intensity measured by flow cytometry.
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Because of the different pH stabilities of the green and red fluorescent proteins, the GFP-LC3 loses its fluorescent signal within the acidic lysosomal environment (pH below 5), but the mRFP-LC3 signal persists.
One possibility is that these GFP-like genes have lost their fluorescent capacity but are retained in the Nematostella genome for other unknown functions.
However, these cells also gave rise to rapidly dividing cancer cells, forming the major tumor mass, which lost the fluorescent signal due to dilution of the dye.
Unfortunately, the ceiling of the General Office is still lost in a fluorescent glare.
As Alexei loses his mind, a dozen other fluorescent and neon beams blink on and off in seesawing, drunken patterns.
The fluorescent LDL imine adduct loses its labeled aldehydophospholipid if exposed to other amino nucleophiles [20].
The excited state loses the energy by returning back to S0 with fluorescent emission or through an intersystem crossing (ISC) process which involves conversion to the long-lived triplet excited state (T 1 * ).
When we hear that she wound up "sitting somewhere where the walls are all white, losing herself underneath those fluorescent lights," we all have a pretty good idea where she is, and it's not in a boring little office somewhere.
Hypericin pre-incubated cells lost 74% of fluorescent Rhodamine123 signal after 20 min of efflux compared to initial amounts taken up (Table 1).
In addition to a background ROI, a representative (adjacent cell) ROI monitored the amount of non-bleached fluorescent signal lost during imaging.
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