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First, an immunofluorescence study using an anti-5-methylcytosine (5mC) antibody showed that chromosome arms lose staining in a replication-dependent way upon the transition from neonatal PSGs to SGs [ 22].
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No amount of money in insurance payouts will bring back a lost stained glass window or other masterpiece.
However, we still recommend taking digital images before mounting when possible, because there is always the possibility that leaf material can suffer mechanical damage or lose stain during the mounting process.
Fisher's exact tests showed that DACH1 expression in MMR- cancers was significantly more likely to be partially/completely lost (staining extension: <70% of cells; p = 0.00016) or relatively weak (intensity scores of <5) (p = 0.054) than that observed in MMR+ cancers.
Finally, clones of enterocytes expressing an active form of Ras lose Armadillo staining at their membrane (Additional file 13D).
These results suggest that amastigotes lose CFSE stain when in the maturing endosomal compartment.
One slide was lost after staining.
During cellular outgrowth, hNPCs differentiate into GFAP+, O4+, and β III tubulin+ glial- and neuronal-like cells while losing nestin staining.
An analysis of FSP1/CD31 double labeling revealed that some cells acquired FSP1 staining and lost CD31 staining, which suggests that the EndMT occurred.
Immunofluorescence with antibodies for the endothelial marker and the mesenchymal marker demonstrated that the HG-treated cells acquired FSP1 staining and lost CD31 staining compared with the control cells.
Some MitoTracker dyes, such as MitoTraker®Orange CMTMRos and MitoTraker®Red CMXRos, are mitochondria membrane potential-sensitive, and they lose the staining ability after mitochondria depolarization; While others, such as MitoTraker®Green and MitoTraker®RedFm, accumulate on mitochondria regardless of mitochondrial membrane potential.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com