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For the LOQ, it was confirmed based on the precision of the peak area.
In order to develop a method with the desired LOQ, it was necessary to use MS-MS detection.
As large sample volumes (up to 1000 µL) of real-world samples including waste and surface waters, some of which were untreated had to be injected in order to achieve the desired LOD and LOQ, it is to be expected that larger amounts of other matrix components, like non-polar organics, humic acids, or transition metals, were co-injected, leading to the decrease in retention time.
However, since the lowest concentration of DHA was exactly the LOQ, it was prone to integration errors.
Although a splitless injection could have increased the LOQ, it would also have decreased the throughput because it would have required a different temperature program to ensure adequate resolution of the solvent peak from the shorter-chain FAMES.
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Only protocol 10 improved slightly LOQ, but since it required additional consumables (Taq DNA polymerase), it is not recommended.
In most instances LoQ exceeds LoD but it's possible for the two quantities to be equal.
In both treatments, the FeDMA concentration reached a maximum: in the treatment with sterilant, the FeDMA concentration gradually increased until 24 h, after which it gradually declined, whereas in the treatment without sterilant, the FeDMA concentration gradually increased until 8 h, after which it dropped below the LOQ between 8 and 24 h after DMA application.
Previous work such as SRMA (Homer and Nelson, 2010) focused on local re-alignment of short reads to improve the accuracy of SNP calls; LoQ uM's broader focus also makes it suitable for improving the accuracy of other types of variant calls.
Considering that this value was established at its final dilution point, it is possible that the true LOQ is higher.
In this study, it was shown that LOD and LOQ are different in terms of quantity using different methods.
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CEO of Professional Science Editing for Scientists @ prosciediting.com