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Among the 16 genes related to cell wall modifications, six encode expansin proteins that are known to have cell-wall loosening activity.
Expansins exhibit wall loosening activity and are involved in plant cell expansion and other developmental events.
Expansin, known to have cell wall loosening activity and is involved in cell expansion and other developmental events during which cell wall modification occurs [ 19], plays important roles during cell elongation [ 20] and expansion [ 21], and specifically regulates fiber elongation [ 22] and fruit ripening [ 23].
α-Expansin and β-expansin proteins are known to exhibit cell wall loosening activity, and are involved in cell expansion and other developmental events; however, expansin-like A and expansin-like B are only known from their gene sequences [ 7], with no experimental evidence about their activity on the cell wall being published [ 8].
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The group-1 allergens from maize pollen, collectively known as Zea m 1, are highly abundant glycoproteins, constituting ∼4% of the protein extracted from pollen; they are rapidly secreted upon pollen hydration and have wall-loosening activity specific for grass cell walls [12], [19].
Both of these grass cell wall components are potential targets of β-expansins in their wall-loosening activity [ 82].
The multi-gene expansin family can be divided up into two main groups, namely, α-expansin (EXPA) and beta-expansin proteins (EXPB), which share very limited (~20%) amino acid similarity even though both are associated with cell-loosening activity [ 6].
Lipid transfer proteins (LTP), one of the most abundant proteins present in the stigma of several species, have cell wall-loosening activity that is similar to expansion, despite its assumed role in lipid metabolism.
The formation of the root hair bulge is thought to involve the cell wall-loosening activity of expansins and coincides with the expression of the EXP7 gene in wild-type roots.
Because their better studied relatives exhibit cell wall-loosening activity and have been implicated in ECM remodeling and the disassembly of ECM structural components, some of these proteins (e.g., expansins and hydrolase) may facilitate the penetration of the pollen tube into the stigma by modifying the papillary cell wall and ECM.
This protein (GrEXP1), a molecule with cell wall loosening (expansin) activity previously seen in plants [ 28] and other organisms with cell walls only [ 29], was found in the plant-parasitic roundworm Globodera rostochiensis.
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