Nevertheless, it may be possible to exploit the predictions as loose restraints to guide the fold-recognition and ab initio simulation procedures.
Namely, loose restraints based on the cryo-ET maps (height = 10 ± 2 nm, width 26 ± 2 nm, inner radius = 8 ± 2 nm) were imposed to ensure the height and width of the assembly to be smaller than, respectively, 10 nm (maximum extension of YscD prior unfolding) and 24 nm (the PrgH rings features a width of about 27 nm).
Other loose restraints were derived from the cross-linking experiments available for the PrgH/PrgK system after sequence alignment with YscD/YscJ (Sanowar et al., 2010; Schraidt et al., 2010), residues between 153 and 160 in YscD were forced to face the YscJ ring and, in particular the region surrounding YscJ-S214.
Third, the rebuilt structure model is refined again with the same parameter settings as the last step of the conservative refinement, but with three different geometric restraint weights: the optimal weight from the conservative optimization phase, a slightly tighter restraint weight and a slightly looser restraint weight.
All quality parameters are constant in the four runs, which means that the application of looser restraints does not degrade the physical relevance of the generated conformations in the sets wNCS_nmrI and wNCS_nmrII.
It was found that the concrete cracking can be mitigated by the proper use of concrete joints, a reasonable construction sequence, early age concrete curing and a loose external restraint.
The isopeptide bond between the two Ubs was defined by imposing a loose distance restraint (10 Å) between the side chain ammonium group of either K48 or K63 and the carboxyl atom of G76.
The debacle at Abu Ghraib shows how badly things can go when average Americans are let loose from those restraints, or allowed to believe that such restraints do not apply to them.
They cut themselves loose from the restraints of tradition, obligation, even family.
A giant inflatable Minion balloon somehow got loose of its restraints and rolled onto a road near the north part of the city, according to the Journal.ie.ie
Overall, if I had to look for an overall score, Edward Scissorhands was quite unique in my life in terms of how I felt about it, feeling very cut loose from all restraints, and yet still, somehow, feeling like I was doing a poor job at the time and have it be the most imitative score of my career.
