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Root cultures were initiated by adding ~500 mg fresh weight of 2-week-old roots into 25 mL of B5 media in a 125-mL flask with a loose foil closure.
Plant cell suspensions were prepared for an experiment by adding 5 mL of 2-week-old suspension culture into 15 mL of MS media supplemented with 0.2 mg/L 2,4-D, 25 g/L sucrose and 2.5 mM KH2PO4 in a 125-mL flask with a loose foil closure and no sponge plug.
Water bath the ramekins, covered in loose foil for about 25 minutes.
The fan inside of the halogen oven is very strong, however, and loose foil will be easily blown off.
Similar(56)
Open foil carefully.
To the specified quantity of solvent in a 250-ml Erlenmeyer flask, add 1 ml of purified n-hexadecane and evaporate on the steam bath under a stream of nitrogen (a loose aluminium foil jacket around the flask will speed evaporation).
Once the nearby Hexagon complex is reborn as a further museum space (a project also being designed OMA, but which is still at a confidential stage), this rough-and-ready shed should make more sense as the loose-fit foil to more conventional galleries planned in the Hexagon buildings.
The Wolverhampton man is the trio's in-house loose cannon; disruptive foil to disciplinarian Matthew Crosby and straight ish) man Ben Clark.
Cook on the lowest shelf of the oven, under a loose covering of foil, for 30 minutes, then lower the oven to 120C and cook for a further 1½ hours.
Cover loosely with foil.
Open the foil.
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