Root cultures were initiated by adding ~500 mg fresh weight of 2-week-old roots into 25 mL of B5 media in a 125-mL flask with a loose foil closure.
Plant cell suspensions were prepared for an experiment by adding 5 mL of 2-week-old suspension culture into 15 mL of MS media supplemented with 0.2 mg/L 2,4-D, 25 g/L sucrose and 2.5 mM KH2PO4 in a 125-mL flask with a loose foil closure and no sponge plug.
However, we did not encounter any major flap venous congestion due to the open bridge technique and non-watertight, loose but secure closure with intermittent sutures.
String can mean either binding in the sense of confining, or closure, tying loose ends.
It's been a tough month for the transatlantic viewer who looks for closure, easy answers and the tying up of loose ends.
At a loose end following HHC's closure, he found himself, for the first time, listening to a selection of old British hip-hop albums purely for fun; realising none of them were available on CD, he had his epiphany.
To circumvent the difficulty and impossibility, we introduce a novel notion of loose-stabilization, that relaxes the closure requirement of self-stabilization; starting from any arbitrary configuration, a system comes to satisfy its specification in a relatively short time, and it keeps the specification not forever but for a long time.
Don't wear French cuffs open and loose without cufflinks or knot closures.
Instead, "[n]arrative nonfiction conclusions return to the intrigue, suspense, or line of inquiry the writer established to draw the reader further into the article, providing a sense of closure and wrapping up any loose ends".
So go inside, tie up loose ends, resolve old issues, and bring closure to things that might be impeding your progress.
