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Using sterile loop of 3 mm diameter, two loopful of culture was added to brilliant green lactose bile broth tubes and incubated at 35°C ± 0.5°C.
About 2 mm loopful of extract, Ag and Au NPs were taken and lowered carefully onto the paper disc.
A loopful of 18 h broth containing organism was transferred to a clean glass slide, Gram stained and examined under microscope.
Completed test was performed by plating a loopful of broth from a positive BGLB tube on to an Eosine Methylene Blue (EMB) Agar plate.
A loopful of the glycerol stock of each isolate was transferred onto nutrient agar, streaked and incubated at 37 °C for 18 24 h.
A loopful of each of five Escherichia coli strains isolated from wound was suspended in 10 ml of sterile distilled water.
A loopful of each of the isolated colonies from M7 agar plates containing heavy oil was transferred to 3 cm diameter wells, containing 5 ml of Luria broth.
Inoculum was prepared by growing one loopful of P. acidilactici culture from MRS agar plate in 250 mL non-baffled Erlenmeyer flasks containing 50 mL MRS medium.
Inoculum was developed by inoculating a loopful of pure culture into a medium consisting of 1 % keratin substrate and 0.2 % yeast extract (pH 7.50).
A loopful of the enrichment culture was streaked onto thiosulphate-citrate-bile salt-sucrose agar used for the selective isolation of Vibrio strains (TCBS agar, Scharlau Microbiology, Spain).
Briefly, first stage culture was initiated by inoculating 25 mL of sterile liquid medium with a loopful of freshly grown culture, under laminar airflow cabinet.
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