Exact(8)
Finally, we validated the importance of the loop score using published data[55], [56].
The central loop score was applied as a filter after all the miRNA MREs were predicted.
Finally, the total loop score was calculated by multiplying the location coefficient with the size coefficient.
The MRE criteria for FindTar version 1.0 includes seed homologies and free energy <−15, and FindTar version 2.0 includes seed homologies, free energy <−15, and loop score ≥20.
The MREs predicted by computational algorithms with or without the loop score were compared with the Gold Standard, the results of luciferase activity assays (Table 1).
Since the central loop of miRNA MRE appeared to be important for miRNA-mediated gene regulation, addition of a loop score as a criterion for MRE prediction should improve the results.
Similar(52)
Website: shopphoenixrising.com 314.862.0609, in the Delmar Loop, scores on all fronts.
The central loop scores demonstrated a significant correlative relationship with miRNA repressive efficiency (Figure. 3B).
The total loop scores were calculated by multiplying the location coefficient with the size coefficient.
Furthermore, the ratios in group screened with the loop scores (L) are higher than those in unscreened group (NL).
The number of predicted MREs in up-regulated genes is then appointed as TU, with loop scores > = 20 as SU.
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