Sentence examples for loop of cells from inspiring English sources

Prior to the aerobic cultivation, yeast pre-cultures were obtained by transferring a loop of cells from the glycerol stocks into 5.0 mL of YNB defined medium (6.7 g L−1 Yeast Nitrogen Base without amino acids (Becton, Dickinson and Company, USA) supplemented with 20 g L−1 d-glucose.

The pre-cultures were recovered by transferring a 10 µL loop of cells from the glycerol stocks stored at −80 °C into 5.0 mL of a growth medium containing 13.4 g yeast nitrogen base (YNB /L, 50 mM potassium hydrogen phthalate buffer at pH 5.5 and was supplemented either with 50 g d-glucose/L or 50 g d-xylose/L, depending on the carbon source used in the further cultivations.

The seed culture was prepared as follows: a loop of cells from the fully-grown slant was inoculated into 50 ml of the above sterile medium in 300-ml Erlenmeyer flasks and incubated for 12 h at 50°C without agitation.

The seed culture was prepared as follows: a loop of cells from the fully grown slant was inoculated into 100 mL of the above medium in 500-mL Erlenmeyer flasks and incubated at 50°C for 12 h with agitation.

β-galactosidase was assayed in a loop of cells put on Whatman paper soaked in 2 ml of Z buffer (Miller, 1972) containing X-Gal (1 g/l) and a drop of zymolase.

A loop of cell colonies of BL21 DE3)/pET20b-torA-gadB was inoculated into 20 mL LB medium in a 250-mL flask and grown overnight.

From the culture stored at 4°C, a loop of cell mass was transferred aseptically to 50 mL YPX media and then incubated in an orbital shaker at 150 rpm and 28°C for reactivation.

A liquid culture was started by adding a loop full of cells from an agar plate to a 250 ml Erlenmeyer flask containing 50 ml of medium.

A liquid culture was started by adding a loop full of cells from a standard agar plate into a 250 ml Erlenmeyer flask containing a 50 ml medium.

A preculture of strain NRRL Y-7124 was inoculated by loop transfer of cells from YM agar to 75 mL ODM + 150 g/L xylose to challenge growth under osmotic stress.

On other occasions, the free trimeric CAK performs functions in the cell cycle, namely, the phosphorylation of the T-loop of cell cycle Cdks (Fisher, 2005).