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b The magnetic loop of PLL-MNPs at 300 K.
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d TEM image of PLL-MNPs.
b f Fluorescence images illustrated the A549 cells treated with different concentrations of PLL-MNPs (25, 50, 100, 200, and 400 µg mL−1).
b f Fluorescence images illustrated the A549 cells treated with various concentrations of PLL-MNPs (25, 50, 100, 200, and 400 µg mL−1) for 48 h.
b f The A549 cells labeled with different concentrations of PLL-MNPs (25, 50, 100, 200, and 400 µg mL−1) were cultured for 48 h.
Magnetic measurements of PLL-MNPs were carried out on a vibrating sample magnetometer LAKESHORE-73044, USA) by changing H between +1375 and −1375 Oe.
The results showed that the DNA composition in S-phase is 23.49 % for the unlabeled A549 cells (Fig. 7a) and that the DNA composition in S-phase of the PLL-MNP-labeled A549 cells with different concentrations of PLL-MNPs is 26.35, 23.87,27.95,24.02, and 26.44 % (Fig. 7b f).
b f The A549 cells treated with different concentrations of PLL-MNPs (25, 50, 100, 200, and 400 µg mL−1) were cultured for 48 h in an RPMI-1640 medium.
The cell cycle of PLL-MNP-labeled A549 cells was analyzed by flow cytometry.
The PI and FDA double-staining protocol was used to detect the cell viability of PLL-MNP-labeled A549 cells.
Open image in new window Fig. 8 Fluorescent staining of PLL-MNP-treated A549 lung cancer cells.
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