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Developmental studies looking for expression of LOX-1 in murine and human placentas have described higher expression of LOX-1 during early to midgestational stages than late gestation [ 78].
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To look for the presence of interferon α mRNA expression we used IFNA1 and IFNA2 RNA in situ probes to look for expression of interferon α mRNA.
To evaluate the expression of ISGs in response to C. pneumoniae infection, we harvested total RNA from cells receiving bacterial or mock infection for the indicated time intervals and looked for expression of CCL5, ISG56, and CXCL10.
Thirdly we have looked for expression of only one viral protein: EBNA-1, ideally we should have investigated for multiple proteins present in different forms of viral latency seen in other EBV associated tumors.
In addition to these molecules, we looked for expression of cytokines, chemokines, and interleukins as a surrogate marker for the potential of these cells to mount an inflammatory response.
We next looked for expression of genes associated with the naive state of pluripotency.
Twenty-four hrs after incubation both the cells were harvested separately and looked for expression levels of cytokines (results are not shown).
We then looked for expression of the corresponding transcripts in the pairwise comparison ovary versus testis in each species (supplementary table S11, Supplementary Material online).
We looked for expression of all relevant constituents of these four major signaling pathways in the airway epithelium by RT-PCR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com