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Based on the hypothesis that these novel transcripts could have a role in regulation of gene expression in either the mammalian or insect host or both, we performed Bowtie2 alignments to the human and the vector (Lutzomyia and Phlebotomous) genomes to search for potential targets in these genomes, looking for complementarity.
Based on the hypothesis that leishmania tsRNAs may act as miRNAs or siRNAs in the mammalian or invertebrate host we performed additional Bowtie2 alignments with all reads mapping to leishmania tRNAs against the human and vector genomes looking for complementarity to find potential targets for these potential regulatory RNAs.
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This may demand particular guidelines in the intervention design for confronting arguments and looking for complementarities between arguments, as well as for enriching the value criteria used during class to approach ideas and concepts.
Look for complementarity between apparently sharply differing viewpoints, she suggests.
So, look for complementarity.
As a second approach to support the coexistence of several micrometric domains, we looked for complementarity.
To find putative interaction sites, we look for complementarity rather than similarity/identity.
The miRanda prediction software identified potential binding sites by looking for high-complementarity regions on the 3′UTRs, which is called seed region [23].
So in summary, I think it is very critical to look for shared vision, look for complementarity, and look for abiding trust.
By looking for convergence (same results), dissonance (opposing results), complementarity (supportive or explanatory results, but not the same) or silence (no evidence to support or refute) we will be able to further test and refine parts of the overarching programme theory.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com