A longitudinally oriented probe was used.
In MCK-Cre/cKO muscle fibers, where the collapsed IF networks generated massive, longitudinally oriented desmin-positive protein aggregates in intermyofibrillar spaces (concurrent with a disintegration of sarcomeric structures), mitochondria no longer appeared to be associated with Z-disks but showed deformation and random orientation.
Furthermore, cell-division orientation is longitudinally biased in the anterior region, shifted from the longitudinal to the circumferential direction along the AP axis, and circumferentially biased in the peripheral region.
This additional external smooth muscle coat showed irregular thickness and haphazard orientation, mostly longitudinally arranged but showing oblique fascicles in some areas.
Long-axis orientation was longitudinally biased in the anterior region and circumferentially biased in the peripheral region (Fig. 5B).
Plotting the absolute value of θ clearly revealed that the long-axis orientation was longitudinally and circumferentially biased in the anterior and peripheral regions, respectively, from 26 to 58 hpf (Fig. 5D), suggesting that the long-axis orientation is spatially regulated in zebrafish lens epithelium.
Outer layer bundles are oriented longitudinally.
Furthermore, cell-division orientation was biased longitudinally in the anterior region, shifted from longitudinal to circumferential along the AP axis, and was biased circumferentially in the peripheral region, suggesting that cell-division orientation is spatially regulated in lens epithelium.
Interestingly, cell-division orientation was biased longitudinally in the anterior region, shifted from longitudinal to circumferential along the anterior posterior axis of lens sphere, and was biased circumferentially in the peripheral region.
Examples are parallel rows of longitudinally aligned platelets, with the orientation extending over several cells (e.g., in Convallaria majalis, shown in Fig. 7i), or rosettes, in which the arrangements of platelets are more or less in radially assembled clusters.
