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The recovered samples were longitudinally cut into halves (i.e., along the axial displacement direction).
The results showed that the mechanical properties of the transversely cut specimens is affected more by the increase in temperature than the longitudinally cut specimens.
We then performed ex vivo tests on longitudinally cut aortas coupled to a loading cell for force measurements with the Extroducer, scaffolded by iron tubing (fig. 2a).
The harvested pellets were fixed in 10% neutral buffered formalin, embedded in paraffin, and longitudinally cut into 4- or 5-µm thick cross sections.
As expected, en-face oil red 'O' staining of longitudinally cut aortas revealed that HF diet induces numerous atherosclerotic plaques not only at the brachiocephalic or curvature areas of the thoracic region, but also all over the abdominal aorta (Figure 1A).
Embedded sites were then longitudinally cut at 5 to 7 μm using a microtome (MICROM®, France).
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Figure 3(B) shows a longitudinally cut-away view of the 3D volumetric OCT image of the 6 mm long rabbit esophagus segment, while the 2D fluorescence intensity map is inlaid on the inner surface of the OCT 3D image.
Flushed colons were opened longitudinally and cut into 2 cm sections.
Fetal small intestines were opened longitudinally and cut into small pieces prior to dissociation with 2 mM EDTA.
The aorta was opened longitudinally and cut into 3-mm long sections and plated on a matrigel pre-coated dish.
Ureter samples were trimmed from fat and connective tissue, opened with fine scissors longitudinally and cut into segments of approximately 2 3 mm for explantation.
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