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Consecutive sections of the tumors co-transplanted with SSc-derived monocytic EPCs confirmed lateral connection of blood vessels detected as a longitudinal vessel section in the single section.
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Quantitative wall measurements were made from vessel cross-sections cut 90° perpendicular to the longitudinal vessel axis from ultrastructural montages taken at ×1.5 2.5 k at 16 megapixel resolution.
Additionally, the longitudinal vessel tension was measured.
Immunostaining density of the CD31+, Podoplanin+ or Lyve-1+ vessels (0, no vessel section; +, 1 to 4 vessel sections; ++, 5 to 19 vessel sections; +++, 20 to 49 vessel sections; ++++, more than 50 vessel sections) was evaluated by light microscopy (x20 objective).
The similarity matrix was examined in two ways to describe longitudinal vessel remapping.
These transverse sections (three sections from each meniscus) and longitudinal sections (three sections from each meniscus) of OA and normal menisci were stained with alizarin red.
The final vessel sections were stained with hematoxylin eosin.
The recipient vessel sections distal to the graft anastomosis were regarded as separate vessels.
It is immediately posterior to the SAZ and connected to the ventral and dorsal longitudinal vessels.
For this reason, they could not explain how the connection was established between the lophophoral and longitudinal vessels.
Embryos that contained a DA, PCV, dorsal longitudinal anastomotic vessel, intersegmental vessels, intersegmental arteries and a parachordal vein as determined by confocal microscopy were scored as normal.
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