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Single gene knockdowns reveal that beetle Robos have specialized axon guidance functions: TcRobo is dedicated to midline repulsion, while TcRobo2/3 also regulates longitudinal pathway formation.
In Df 3L HD1 embryos (Figure 2G), axons in the inner 1D4 longitudinal pathway cross the midline.
We also observed guidance errors with anti-FasIII in which RP axons turn anteriorly along the longitudinal pathway.
(A – E ) Stage 17 embryos carrying elav-GAL4 and the indicated UAS-Robo transgenes, stained with anti-HRP (magenta) and the longitudinal pathway marker anti-FasciclinII (FasII; green).
Childsmile aims to improve the oral health of Scotland's children through a comprehensive, longitudinal, pathway of care delivered in clinical and community settings [ 5]. 'Childsmile Practice', a key component of the Childsmile programme, promotes a shift from 'reactive management' to 'anticipatory care' [ 5].
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The overall architecture and arrangement of longitudinal pathways are remarkably conserved in Tribolium, despite it having only two Robos.
In contrast to dorso-ventral navigation, no major general guidance cues required for the establishment of longitudinal pathways have been identified so far.
Most CNS growth cones initially head straight towards the midline, and only after crossing, they change their behavior as they turn and follow specific longitudinal pathways.
(A ) Embryos carrying elav-GAL4 alone exhibit a wild-type arrangement of axon pathways, including three FasII-positive longitudinal pathways that do not cross the midline.
Structural equation models using latent variable paths are appropriate techniques for charting longitudinal pathways (in our case between alcohol (mis)use and antisocial behaviour).
(A ) Embryos carrying elav-GAL4 alone exhibit a wild-type arrangement of axon pathways, including distinct anterior and posterior commissures and three FasII-positive longitudinal pathways that do not cross the midline.
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