Epidermal thickness was analysed by measurement of the interfollicular epidermal area and corresponding length on H&E stained longitudinal paraffin sections (7 µm) from mouse back skin.
Longitudinal paraffin sections were stained with hematoxylin and eosin (H&E), May Grünwald Giemsa or immunostained with anti-von Willebrand Factor (Dako, Agilent technologies, Santa Clara, CA).
(F ) Longitudinal paraffin sections of the thoracic aorta from Vegfr3 fl/fl (WT) or EC iΔR3 mice or EC iΔR3 mice, probed for MMP9 (blue) and nuclei (red) after injection of Tx for the indicated time (WT is 1 week post-injection).
Histopathological examination of longitudinal paraffin-embedded sections of the sciatic nerve 5 days after the sixth weekly injection revealed degenerative changes.
Longitudinal paraffin or frozen sections were incubated with the following antibodies: anti-S100 (Dako), anti-MIB1 (Dako), B220/CD45R for B-cells (Pharmingen), CD3 for T-cells (clone SP7, NeoMarkers), CD68 for macrophages (Serotec) or stained with haematoxylin-eosin.
a paraffin section of hull in CJ06.
e paraffin section of hull in TN1.
i paraffin section of hull in CSSL1-2.
C, E. Paraffin section of flag leaf of PA64s.
B, D. Paraffin section of flag leaf of 93 11.
