Long-term relative competent vector population density resulting from release scenarios that arise from different combinations of release ratio and release duration with male-only (A,D), bi-sex (B,E), and female-only releases (C,F).
R&R-only releases are often less effective at long-term reduction of competent vectors than AP-only releases or R&R/AP releases.
In most of the male-only release scenarios that we analyzed, the AP-only releases led to the most effective long-term reduction of competent vectors.
Long-term maintenance of competent yellow type embryogenic callus was achieved by sub-culturing it every month on fresh gelled proliferation media.
Bi-sex AP-only releases caused greater long-term reduction in competent vectors than similar male-only and female-only releases.
The exception was that for very weak density dependence, FK/AP releases led to lower long-term density in competent vectors than AP-only releases, while for stronger density dependence, AP-only reledses led to lower long-term density of competent vectors than FK/AP releases (Fig. 3B,C).
In this paper, we showed that vector control strategies involving strains with FK and AP genes, individually and in combination, can have very different impacts on transient and long-term dynamics of competent vector populations.
The period of reduction left less time for AP releases, resulting in a higher long-term density of competent vectors than when AP-only releases were conducted throughout the entire release duration.
We note that the differences between the long-term density of competent vectors following AP-only, R&R/AP, and FK/AP releases were small when the switch time was earlier.
For weak density dependence, R&R led to the greatest reduction in competent vector density during the transient period while AP and FK/AP releases led to the least reduction; R&R/AP releases caused the greatest long-term reduction in competent vectors and R&R-only releases caused the least long-term reduction when density dependence was weak (Fig. 3A).
