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To overcome poor infection efficiency and other defects, we used the lentiviral vector as a delivery tool for siRNAs to silence genes, which had advantages in the large capacity of transfer gene fragment, a long time expression of target gene and little immune response.
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Although the prognostic significance of proliferation in early invasive breast cancer has been recognized for a long time, recent gene-expression profiling studies have reemphasized its biologic and prognostic value and the potential application of its assessment in routine practice, particularly to define prognostic subgroups of luminal/hormone receptor-positive (HR+) tumors.
For long incubation times, expression of cleaved caspase-7, -6 and PARP was obviously higher and enzyme cleavage occurred at lower light doses (LD63 for 24 h vs LD93 for 3 h).
He paused for a long time, searching for the expression that would do justice to the whole business.
The nmt1 promoter-dependent overproduction required a long time (12 15 h) before expression.
It has been used for a long time to study gene expression, nervous and circulatory systems, cardiovascular disease and cancer, and thus have received intense attention [ 2– 4].
He pointed out that this linguistic mentality helped create such expressions as "long time no see," a word-for-word translation of a Chinese expression that became a mainstay of spoken English.
Zip over to Poland, and there's Maceba, who, according to owner Bozena Wrona, sometimes "just pounces on the canvas and paints for a long time with a lot of expression," but other times is just not "in the mood".
Perhaps not, if it is allowed to linger in the genome for a long time despite partial loss of expression and accumulation of harmful mutations.
For species that have diverged for sufficiently long time no detectable similarity in expression is expected to remain; this has been postulated to be the case between mouse and human (100 million years; Jordan et al., 2005).
It has been known for a long time that in eukaryotes gene expression involves regulation at the level of transcription and post-transcription, including maturation, transfer of the mRNA into the cytoplasm, as well as translational and post-translational regulation and also at the level of protein half-life.
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