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We then analyzed PSA-NCAM expression in a human GBM cell line GBM9, previously developed in our laboratory from a patient biopsy and characterized by a highly proliferative behavior and long term sphere formation [ 9].
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To determine whether these spheroids can be expanded in vitro, the spheroids were dissociated into single cell suspensions and passaged multiple times in long-term sphere-forming assay.
It will cause a more blurred university-industry boundary if the URE stays in the core of the university institutional sphere long term.
Similarly, long-term cultured sphere forming cells, whatever established from CD133+ or CD133- tumor cells were equally resistant to temozolomide.
Freshly isolated CD133+ colorectal cancer cells gave rise to long-term tumour sphere (or spheroids) cultures maintaining CD133 expression.
According to Sphere CEO Tony Conrad, the test performed so far above expectations that About.com has agreed to a long term deal to license the technology.
Within a media sphere dominated by short form content, is it possible to create short form content that actually creates long term response?
"Think long term," he said.
It's very long term.
Long term?
What about long term?
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CEO of Professional Science Editing for Scientists @ prosciediting.com