Long term passages of these cell lines in different laboratories may cause them to differ significantly from the original patient cells, and new models closer to prostate tissue are desirable.
Karyotype was assessed during the process of establishment and long-term passage of rES cells.
The granulosa-cell line KGN, which was derived through long-term passage of a recurrent A-GCT [5], was the only cell line found to harbor the mutation.
Long-term passage of iPSCs has been found to be able to diminish the epigenetic signature inherited from the parent cells [100].
The long-term passage of the culture demonstrated consistent, sustained, logarithmic cell growth, indicating that these culture conditions support GSC cell divisions.
OV90, derived from a long-term passage of undifferentiated adenocarcinoma of malignant ovarian ascites, exhibits the molecular genetic characteristics of HGSC, which includes the presence of a somatic TP53 mutation and complex genomic rearrangements overlapping the spectrum of anomalies observed in HGSCs [ 15, 19].
As a result, many subcultures were performed, and the strains designated as "Hall" strains may not be identical to or may differ from the original isolates as a result of long term passage.
Long-term passaging of MM cells and patient serum as an essential component in the NBM.
The EOC cell lines were derived from long-term passages of tumor tissues representing different histopathological subtypes of ovarian cancer [ 2].
Because of the high frequency of genomic mutations during long-term passaging of ESCs and iPSCs, it is necessary to determine whether hUC-MSCs, such as ESCs and iPSCs, can be grown indefinitely in culture and undergo adaptive genetic changes during the long-term expansion.
Briefly, lenalidomide-resistant H929 cells were made by continuous treatment with lenalidomide, and DF15 cells were made resistant to pomalidomide by long-term passage in the presence of pomalidomide.
