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The clonogenic assay is a long term assay but technical drawbacks and the long culturing time have limited the clinical use of the method [ 9, 10].
Ovarian samples were collected either at 6, 12, 24 hours post injection for short-term assay, or at 7, 15, 23, 30-day after injection for long term assay.
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To determine which signaling pathway(s) may be involved in CSPG's effect on NSC survival we carried out both short and long term assays.
It has been argued that, in settings of high endemicity where a mixture of recent and old infections are commonly found, long term assays are more sensitive than those with shorter culture times [34].
The therapeutic endpoint in p53 −/− tumor cells has to be clarified using long term assays and in vivo models.
These data indicate that the same tumor cells whose sensitivity to etoposide is not affected by FADD-DD or the V108E mutant in vitro (in both short- and long- term assays), do display reduced chemosensitivity in vivo.
For the long-term assay, 85 female ICR mice were given N-methyl-N-nitrsourea solution into their uterine corpora.
In the long-term assay, we used the well-established induction of senescence by RB in the RB-deficient human SAOS-2 cells.
We next measured the growth suppression activity of RB mutants by a short-term assay of BrdU-incorporation and a long-term assay of flat cell formation.
In the long-term assay the same stimuli were applied at the beginning except for SEB that was added on day 6 to a well of non-stimulated cultured cells.
In the long-term assay, brefeldin A (10 µg/ml) was added 4 h after the second restimulation only, and cells were then cultured for an additional 12 h.
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