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Vitamin D levels did not change in the non-survivor group from day 1 to day 5 (5.3 ng/ml to 5.7 ng/ml, p = 0.89).Kaplan Meier survival analysis revealed that patients with vitamin D levels ≥ 6.8 ng/ml (median value) had increased 28-day survival as compared to patients with low vitamin D levels (<6.8 ng/ml) (long rank test p = 0.012).
By long rank test, survival probabilities were significantly low at these strata.
The long rank test was used to assess presence of significant difference in survival status of EBF between rural and urban.
Moreover, we found that the Bmi-1 gene expression and high- or low-grade tumors were predictor of survival as evidenced by Kaplan-Meier curves and long rank test.
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Weaning time and rate of successful weaning were not different in the protocol group compared to the control group (long-rank test, p = 0.31 for MV duration, p = 0.45 for weaning time).
The overall survival curves were calculated using the Kaplan-Meier method and analyzed using the long-rank test.
CFU counts were analyzed with non-parametric Wilcoxon Rank sum tests, while mean survival times were compared among groups by using the long-rank test.
On the other hand, time to survival data in experimental metastasis assay were analyzed by Kaplan-Meier method and compared by the long-rank test.
From prognosis analysis, we found that higher levels of infiltrating CD8+ CTLs or CD57+NK cells correlated with a better overall survival of ESCC patients (P<0.001 and P = 0.002, respectively, determined by long-rank test, Figure 5).
The overall survival curves of the patients in this study are depicted in Figure 2. The statistical analysis demonstrated a positive correlation between overall survival and the density of IL-17+ TILs (Figure 2, long-rank test: P = 0.031).
Long-rank test was used for the analysis of significance.
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