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The large size of BACs means that they can generally carry intact genes with all the long range controlling elements that drive full levels of tissue-specific expression.
BAC clones containing entire mammalian genes including all the transcribed region and long range controlling elements are very useful for functional analysis.
The large insert size of the clones allow many mammalian genes to be cloned intact with all the long range controlling elements and BAC transgenics have generally given physiological levels of tissue specific expression in transgenic mice (for example [ 1, 2]).
For gene delivery and expression in tissue culture, or for gene therapy, the large inserts of the BACs are very useful as they generally contain all the long range controlling elements needed for full levels and tissue specific expression of genes.
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The impairment of long range control underlies a variety of severe human disorders [2].
The callipyge mutation has altered a putative long range control element [17] but has not altered the parent-of-origin allele-specific expression [10].
We assume that long range control of transcription, involving IG1 or introns, should have a role in the regulation of deimination.
These results support the hypothesis that the mutation has disrupted a long range control element [ 27].
In the animals with a paternally inherited callipyge allele, (+ Mat / CLPG Pat and CLPG Mat / CLPG Pat ), the normal repression of the PEG11 locus has been disrupted by the mutation in a putative long range control element [ 27].
The question, then, of course, is whether agents can exercise indirect long-range control over their implicit biases.
This suggests that we can be held responsible for attitudes or behaviors over which we only have indirect long-range control.
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