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The predicted BVMOAf1 contains 905 amino acids comprising an N-terminal BVMO domain (~540 residues) fused to a ~360 amino acids long putative polynucleotide synthase domain.
The exon 1 encodes for a 23 amino acids long putative signal peptide which is likely to be cleaved off whereas the exon 2 encodes for the mature protein of 252 amino acids.
num.: KT160228; pyrophosphatase gene: 2795 bp long transcript, 475 aa long putative protein, GenBank acc.
num.: KT160227; adenosine deaminase gene: 2180 bp long transcript, 508 aa long putative protein, GenBank acc.
The ORF of PenB_MT2 encodes a 143 AA long putative protein with a calculated molecular mass of 15.03 kDa and a predicted pI of 5.49.
However, the shortest array was positioned within a 423 bp long putative LCRIS_01228 gene and thus is most likely a false prediction.
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As illustrated in Figure 1a, OEP9 (At1g16000) is an 86 amino-acid-long putative TA protein, possessing of a single predicted TMD and a 32 amino-acid-long hydrophilic C-terminal sequence (CTS).
For RMEL2, the longest putative ORF encodes a polypeptide chain of 98 aa residues, whose sequence is conserved within primates.
For RMEL3, the longest putative ORF is an AUG-starting ORF that encodes a 45 aa long polypeptide conserved in the other three primates (Figures 6C and Figure S3).
We performed a simple open reading frame analysis (ORF) on all of the Sm454ESTABI transcripts to identify the longest putative protein that could be coded for by each transcript file (Dataset S5).
Notably, orthologs of one immediate neighbor of Pax10 (an uncharacterized gene encoding 776 amino acid-long putative peptide; ENSACAG00000013620) are also absent from mammalian genomes.
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