Exact(10)
Microsoft has been a long promoter of the concept, sinking billions of dollars into its Surface project.
DNA substrates used in this study include long promoter DNA of three relBE modules and their partial fragments.
Without it, neither the short nor a long promoter was capable of inducing expression.
As a promoter test vector we used the pILA plasmid [ 43] into which ~300 bp long promoter fragments were cloned as transcriptional fusions with the luxAB genes.
HLA-G gene expression is regulated by several factors which are not yet completely elucidated, and both its long promoter region, and its 3' untranslated region (UTR) are supposed to play important roles in this process.
The 770 bp (short) and 2.5 kb (long) promoter regions of miR-373 were cloned into pGL4.10-basic reporter vector, and the mutations of predicted CREB binding sites were made.
Similar(50)
The long (promoter-distal) transcripts are dependent on the elongation stimulatory activity of DSIF.
Nuclear extracts (NE) containing the constitutively active P-TEFb and WT DSIF led to a time-dependent increase of the long (promoter-distal) RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5 (Figure 3A, first three lanes).
Consistent patterns of GFP expression were observed in the brain, accessory gland, wing and leg across all transgenic lines, with noted differences of expression in proboscis between the short and long promoter-GFP constructs (see below).
The elongation stimulation activity of DSIF was assayed using pSLG402 as a template, which generates short (promoter-proximal) and long (promoter-distal) RNase T1-resistant products under the control of the adenovirus major-late promoter.
Long, promoter-associated CGIs are stably hypomethylated across different cell types, while short, intra- or inter-genic CGIs exhibit the most variable patterns DNA methylation.
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