Sentence examples for long primer from inspiring English sources

THIS week's print edition includes a long primer on QE.

Here we present a novel strategy for primer design, central overlapping primers (COP), which employs a pair of bipartite primers of different lengths, with the short primer complementary to the middle region of the long primer.

Sequences of these regions were then mapped to the human and chimpanzee genomes to verify their uniqueness using Primer-Blast (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). Primers were designed using Primer3 [36] with the following parameters: 100 150 base pairs (bp) product long, primer Tm min 58°C, opt 59°C, max 61°C; primer size min 17 bp, opt 20 bp; max 23 bp.

However, it was possible that, because the FR primers used in the experiment were long (5' primer: 19 bases; 3' primer: 21 bases), some of the bases on the 5' end of the primers may not have bound with the template DNA.

NV3Dpol also showed a weak activity of elongation-reaction from a long primer.

Alternatively, specific DNA fragments can be separated from long primer dimers (>100 bp) or excessive unspecific PCR products by gel extraction.

To achieve this aim, we designed long primers and used nested inverse PCR to amplify the DNA flanking sequences.

Briefly, individual barcoded libraries are directly prepared by PCR using long primers incorporating the Illumina adapter sequences which allow large numbers of libraries to combined and sequenced on Illumina sequencers.

A first amplification reaction (first PCR) served to enrich the proper 16S rDNA template, which was not achieved when using long primers as those used in the following steps.

This methodology includes a two-step PCR reaction in which overlapping long primers allow for the complete generation of pre-amiR319e-GFP molecules that are adequate for recombination into Gateway vectors with no further requirements.

Synthesis of the latter transcripts is dependent on 8 15 nt long primers derived from the 5'ends of capped endogenous mRNAs.