Sentence examples for long polymerase from inspiring English sources

Second, the entire plasmid was randomly mutated in a slightly mutagenic long polymerase chain reaction.

Partial sequences of the mitochondrial genomes were determined for two calanoid copepods, Eucalanus bungii [9530 bp (base pairs)] and Neocalanus cristatus (7965 bp), using an approach that employs a long polymerase chain reaction (PCR) technique and primer walking.

For the RT-PCR we used SequalPrep Long Polymerase (Invitrogen), a forward primer located in exon 2 (5'-GCGGAGTCATTCAGTAGCCATCTT-3'), and a reverse primer located in the 3'-UTR of exon 20 (5'-AGTTGGAAGGTCTTCCTGCAACAG-3') to amplify the 2521/2606/3726/3811 bp products containing the entire MFN2 open reading frame.

Long polymerase chain reaction (PCR) primers containing the mutations were used and the mutated fragments were cloned in the SMG4-gal4 vector.

Both Timmermans et al. (2010) and McComish et al. (2010) sequenced long polymerase chain reaction (PCR) amplified fragments covering the entire mitogenome.

Because the mtDNA preparation of H. aroides was considerably contaminated with nuclear DNA (which is nearly as A + T-rich as mtDNA), random sequencing was combined with sequencing of DNA amplified by long polymerase chain reaction.

Attempts to amplify the genome in several 5−6 kb pieces using a long- polymerase chain reaction (PCR) protocol (Burger et al. 2007) were unsuccessful due to the relatively poor quality of the DNA template.

Inside the kit there are a ready-to-use Master Mix (DM Master MIX), an Extra-Long Polymerase (DM DNA Polymerase), digoxigenin-labeled probe, DNA Molecular Weight Markers VII and VIII, DIG labeled (Roche Diagnostics), and step-by-step instructions and suggestions for optimization the analysis.

We isolated Rivulus marmoratus mitochondrial DNA by long-polymerase chain reaction with conserved primers, and sequenced it with 36 sets of internal conserved primers, which were designed from the extensive sequence similarities of mitochondrial DNA from several fish species.

For this reason, in this work we developed a new molecular diagnostic assay, Myotonic Dystrophy SB kit, a standardized and certified method, based on the combination of Long-Polymerase Chain Reaction and Southern Blot Analysis SBAA), to better characterize the DM1 mutation in a cohort of clinically well-defined DM1 patients attending the Neuromuscular Clinic at IRCCS Policlinico San Donato.

The presence of the 13 kbp ACSSuT resistance gene cluster was determined by a long-polymerase chain reaction (PCR) method developed by Walker et al. (7) that amplified the 10- kbp region between the aadA2 (streptomycin resistance) and bla CARB-2 (ampicillin resistance) genes on the sequence of the antibiotic resistance gene cluster from S. Typhimurium DT 104 isolate H3380 (8).