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Among these, we selected three gene pairs that, according to the transcript annotation on TAIR [18] have long overlapping regions.
Briefly, the entire mtDNA.sequence was amplified in 3 long overlapping PCR fragments, with each reaction containing 50 ng of genomic DNA.
The peptide array consisted of 66 synthetic 16 28 amino acids long overlapping peptides, spanning amino acids 1607 1989 of the P. falciparum-FCR3 var2csequencence (Table S1).
Oligonucleotides (102 107mer long) overlapping by 20 base pairs and representing the polyepitope DNA sequence, were annealed together by using Splicing by Overlap Extension and stepwise asymmetric PCR [16].
Long overlapping reads, together with increased depth of coverage, make genome reconstruction less challenging.
Additionally, the cassette has long overlapping regions of immunoglobulin gene-specific homology on its ends (VP1VT1 and VP2VT2).
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Long overlapped, at the end of his career, with Justin Strzelczyk, who was also found to have C.T.E.
Pastoralists have long overlapped with wildlife in rangelands of sub-Saharan Africa.
In this case, 9 to 10 residue-long overlapping domains between adjacent molecules untwist relative to each other to accommodate orthogonal interactions between chains in the junctional four-helix nexus.
The nearest matching starting time strategy and longest overlapping time strategy obtained 89.7% and 83.5% association rates, respectively, and thus, our method was proven to function effectively.
We therefore trimmed each sequence, leaving a 580 bp-long overlapping COI fragment and a 451-455 bp-long 16S fragment.
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