Exact(1)
Long Duration Live Imaging: 1 3 Females were dissected into halocarbon oil 700 (Sigma), and individual stage 13 oocytes were transferred to fresh halocarbon oil on a well slide (made by placing a square border made of electrical tape on a no. 1 1/2 cover slip) using a micro-hook.
Similar(59)
In an interview with the elderly, the elderly also feel that this pattern is better than long duration living together, "This is the only solution, we certainly want to have our own freedom and living space, but working and taking care of kids at the same time is also hard for our children, so we had to help".
You may be travelling with such one for long durations, living in close quarters or other such circumstances that warrant your female close protection agent to have a decent personality.
However, using our standard protocol for long duration live-imaging analysis of oocytes [24] preparations are covered in an oxygen-permeable membrane, and the time required for sample preparation meant that imaging did not begin until ∼20 minutes after anesthetization.
Among the methodological differences between the previously published live imaging of the GFP-Polo line and our standard long duration live-imaging protocol were the steps taken to prolong oocyte viability.
Thus, long-duration live action shots were not used.
Using our standard protocol for long-duration live-imaging [24], we examined living oocytes expressing either GFP-Mps1 [16] or GFP-Polo [25] that would be expected to have completed NEB based on dorsal appendage development [2].
This failure to observe oocytes in long-duration live-imaging was unexpected, as approximately 70% of fixed prometaphase oocytes contained filaments, and our previous study had readily observed GFP filaments with both fixed oocytes expressing GFP-Mps1 and in live oocytes expressing GFP-Polo.
Similar effects may have taken place with regard to exposure to passive smoking although the information concerning long duration of living with a smoker has probably not changed much during follow-up.
It didn't take long for me to sense that Cage played a major role in setting the tone for period attitudes in the nineteen sixties about the incommensurability between recording and various kinds of experimental music that began in that period: indeterminate musics, text scores, long-duration minimalism, live electronic music, free improvisation, and so on.
The deleterious effects of accelerated heavy ions on living cells are of increasing importance for long duration human space flight activities.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com