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This resulted in a 1539 bp long alignment for the 16S rRNA sequences, and a total of 150387 aligned amino-acids for the protein sequences.
The tunnels are characterized with high overburden, long alignment and complex geological conditions.
Considering Sanders' long alignment with the labor movement, "he can talk about our issues as well as we can talk about our issues," DeMoro told reporters. .
The route was split twice since 1953, first by the removal of the old Mullica River bridge in 1962 as part of a sale to the National Park Service for a refuge in Virginia, then the northern portion was dismantled except for a 0.12 mile long alignment for a wetland mitigation project.
There was an artificial platform there and this apparent verification of Thom's long alignment hypothesis (Kintraw was diagnosed as an accurate winter solstice site) led him to check Thom's geometrical theories at the Cultoon stone circle in Islay, also with a positive result.
The alignments were then concatenated and the resulting long alignment was used to construct a phylogenetic tree.
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Considerable parts of the ITS were not identically aligned between these three approaches, including a very long alignment-ambiguous part of the ITS1 and two shorter alignment-ambiguous parts of the ITS2.
The resulting contiguous sequences (contigs) were aligned to hg18 using BWA version 0.5.7 for long alignments with parameter "-r 1".
Our alignments validated these difficulties in that we found ITS sequences to be highly divergent across a wide taxon sampling, with long alignments and comparatively high percentages of parsimony-informative positions.
Hence, our approach will not scale to long alignments containing many gaps.
On long alignments, fortunately, the chance of missing all seeds is small.
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