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Biocular, one minute long acquisitions were obtained from 13 young, healthy subjects (aged 27.1 ± 6.9; 5 females, 8 males).
However, as mentioned when presenting the experimental setup, acquisition chains like TCSPC are limited with a maximum count rate, which requires long acquisitions in order to increase signal dynamics.
Single-molecule fluorescence techniques are powerful tools for observing molecular conformations, interactions, concentrations, and motion [ 1, 2] but since they must operate at the single-molecule level, they generally require long acquisitions to acquire adequate statistics.
3D MR long acquisitions depend on the subject's condition and are very motion sensitive, and in consequence, this 3D approach is limited in clinical practice when subjects are patients with neurodegenerative diseases who cannot remain in a stable condition during a long acquisition and who often present movement and shaking troubles.
As the proposed approach is based on a short acquisition protocol and showed good performance even in a small-size group, it may be easily adapted to clinical studies when it is not possible to acquire large datasets (e.g. in rare diseases such as leukodystrophies) or when the patients cannot withstand long acquisitions (e.g. psychiatric patients).
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PPR, for one, was said to be interested in making a long acquisition.
Such long acquisition time is required to increase spectral quality, especially for relatively weak signals.
For such a long acquisition time, the camera is likely saturated by its dark current.
Such algorithms avoid the long acquisition transients encountered with feedback algorithms.
A disadvantage of the SPACE sequence is its long acquisition time (10 min 4 s).
We have also demonstrated that some holes will remain undetectable by an observer, even after extremely long acquisition times.
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