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All inoculated foxes, except fox 5FE-45F8 (receiving 3.7 logs of virus), 5FE-60DD (3.0 logs) and 5FD-ACB1 (3.0 logsurvivedved the challenge and did not develop clinical signs of rabies or signs suggesting encephalitis, during the 13 months of observation.
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Cytotoxicity was quantitated by linear regression analysis of plots of log surviving fraction vs TMZ dose to obtain the dose required to reduce survival to 10%, LD10.
The decimal-reduction times (D-values) were calculated by linear regression analysis (D=−1/slope of a plot of log surviving cells versus time).
The correlation of determination, R2, between the observed log surviving fraction (LSF) of cells from each of the conditions studied in the experiment, for the overall model was 0.811.
Log (surviving fraction) was plotted against dose for each condition.
A plot of growth delays against log surviving fraction was linear below a fraction of 0.03, but failed to extrapolate to the origin.
In three 0.25-ha plots, we measured annual in situ net N mineralization in mineral soil using resin cores (n = 100 per plot) under pine saplings, downed wood (legacy logs that survived the fire, and fire-killed trees that had fallen and were contacting or elevated above the ground), and in bare mineral soil.
All patients with high maximum Oxa-51 DNA (>4 log copies/mL) during empirical therapy died within 17 days, whereas 70% of patients with low maximum Oxa-51 DNA (<2 log copies/mL) survived (P = 0.003, log-rank test) (Figure 4A).
The percentage survival of the bacteria was calculated as follows: (1) % survival = log cfu of viable cells survived log cfu of initial viable cells inoculated × 100.
Studies were therefore undertaken to find out whether their propagules are able to colonise bark of Pinus radiata logs or survive on the bark surface long enough to be carried overseas.
All six EBLV-1 inoculated foxes, except one (fox 634-8FE1 receiving a viral dose of 3.7 logs MIC LD50), survived the challenge till 20 months p.i. and did not develop any clinical signs indicative of rabies or suggestive of encephalitis.
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