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CEUS was performed with a Siemens Acuson, Sequoia 512 equipment, using Sonovue (Bracco) as an enhancing agent with a compatible logistic program.
Logistic regression including the studied parameters of the univariate analysis was performed using the logistic program of SPSS 10.0®: a forward regression procedure was used with a P value <0.05 for entry.
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Dr. E. Remarque kindly provided a copy of his logistic titration program.
A detailed method, the input files, output files and the logistic regression program used in this study are available in [ 53].
Concentrations were calculated by comparison with known PGE2 standards using a 5 parameter logistic curve fitting program.
For logistic regression this program uses the methodology described in Hsieh, Block & Larsen's 1997 paper [ 28].
Dose response data were analyzed with a four-parameter logistic curve-fitting program (JMP Statistical Analysis software; SAS Institute Inc., Cary, NC, USA).
The optical density is used to calculate the concentration of the biomarker by using the Softmax Pro 4.7.1 software utilizing a 4-parameter logistic curve fitting program.
In addition, multivariate logistic models were programmed.
Standard curves were generated for each cytokine in concordance with the manufacturer's instructions and the concentrations of unknown samples were calculated using a 5 parametric curve-fitting program with logistic regression Bio-Rad Laboratoriess, Hercules, CA).
There is a need to strengthen the current logistic system of LHW program and the health department at district level.
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