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Only genes with p ≤ 0.05 and log ratio of at least 2.0 were taken for upregulation, and a log ratio of 0.5 was considered for downregulation.
Fold difference was calculated from the estimated log ratio.
PPLR associates probability values (between 0 and 1) to each log ratio, which represents the probability of the log Ratio being positive.
A quantitative estimate of the change in gene expression was given by the signal log ratio, and the fold increase was calculated as fold increase = 2 log ratio).
The "absolute depletion" ("A" for short) measures the signal level (log ratio) at the bottom.
After normalization the Log ratio, Log2(mutant/control), for each feature on each array was determined.
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PLR: parametric log-ratio model [6].
Four log-ratio transformations, including additive log-ratio (alr), centered log-ratio (clr), isometric log-ratio (ilr), and symmetry log-ratio (slr), combined with ordinary kriging (log-ratio kriging: alr_OK, clr_OK, ilr_OK and slr_OK) were selected to be compared with compositional kriging (CK) for the spatial prediction of soil psf in Tianlaochi of Heihe River Basin, China.
As aforementioned, we decided to do a log-ratio scaling.
Compositional data analysis using centered log-ratio (clr) or isometric log-ratio (ilr) coordinates avoids such difficulties and preserves sub-compositional coherence in the analysis.
The results show that using isometric log-ratio (ILR) transformation with different interpolators can achieve relatively better performance than the other log-ratio transformation methods.
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