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When the plasmid carrying insertion sequence IS 900 was applied for the amplification, the linear regression of the qPCR assay resulted in an amplification efficiency of 97 % and a linear dynamic range spanning 8 log dilutions (quantification cycles [Cq] from 14.34 to 38.96), with an R of 0.9989.
Notch family (Notch1 4, Hes1, Jag1, and NF-k β) and TGF- β family gene expressions were studied by real-time PCR, flow cytometry, and immunohistochemistry. Relative expression of Notch signaling target genes, Hes1 and NF-k β, was higher in the total PBMCs of AVH-B and CHB patients than that in HC patients (Log relative quantification (RQ); 1.1 AVH-B vs. 0.3 HC, 1.3 CHB vs. 0.3 HC; P=0.02).
The former test had a detection limit of 103 cDNA molecules per reaction with a log-linear quantification range of up to 1011 (R2 = 0.98), while the latter test was able to detect 500 cDNA-BTV molecules/PCR.
Most studies on the carbon balance of selectively logged forests have estimated pre- and post-logging carbon stocks with remote sensing methods [16], allowing large-scale estimation of logging extent and quantification of canopy damage [14, 17, 18].
A standard curve for quantification (log-linear R2 > 0.95) was generated using genomic DNA from Nitrosomonas europaea ATCC 19718 (Chain et al.2003) in a dilution series that spanned from 101 to 109 gene copies per reaction.
ImageMaster 2D Platinum 5.0 software was used for spot detection, gel alignment, spot quantification, and log normalized data, followed by calculation of the protein molecular weight (MW) of proteins in synchronizing electrophoresis and the isoelectric point (pI) over the pH range of the IEF gel strips.
Rad52-yellow fluorescent protein (YFP)− and RPA (Rad1 -green fluoRad1 -greenotein−expressing strains were grown in Edinburgh minimal medium until mid-log phase fluorescenceantification assays.
The comparative ∆∆C q method was used to calculate fold-changes (ΔC q = C q microRNA of interest - C q reference control, Relative Quantification [RQ]=2 -ΔΔCq and LOG transformed = LOG 2RQ).
The results clearly indicate that ATLD are reliable for the simultaneous quantification of GPS, LOG, SWM, and SWS.
Kinetic quantification in the log phase of the reaction, the phase of constant efficiency, clearly generates a larger linear response than end-point detection in the plateau phase of the reaction (Fig. 1).
The slope determined from the log-log plot was further used for lipid quantification (Nair and Werling 2009).
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